RU.521

99.91%

3-(1-(6,7-Dichloro-1H-benzo[d]imidazol-2-yl)-5-hydroxy-3-methyl-1H-pyrazol-4-yl)isobenzofuran-1(3H)-one

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品牌：源叶(MedMol)

产品编号：S89346 一键复制产品信息

CAS号：2262452-06-0

分子式：C₁₉H₁₂Cl₂N₄O₃

分子量：415.23

别名：RU320521；3-(1-(6,7-二氯-1H-苯并[d]咪唑-2-基)-5-羟基-3-甲基-1H-吡唑-4-基)异苯并呋喃-1(3H)-酮

货号	规格	价格	上海	北京	珠海	重庆	武汉
S89346-5mg买3赠1	99.91%	￥580.00 S89346-5mg ￥580.00 现货（买3赠1）	6 4 6	2	2	-	-
S89346-10mg买3赠1	99.91%	￥870.00 S89346-10mg ￥870.00 现货（买3赠1）	6 6	-	-	-	-
S89346-25mg买3赠1	99.91%	￥1400.00 S89346-25mg ￥1400.00 现货（买3赠1）	2 2 2	2	-	-	-
S89346-100mg买3赠1	99.91%	￥4300.00 S89346-100mg ￥4300.00 现货（买3赠1）	2 0 2	-	-	-	-

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产品介绍参考文献(1篇) 质检证书(COA) 摩尔浓度计算器相关产品

产品描述：

RU.521 (RU320521) is a potent dsDNA inhibitor (IC50 : 700 nM), inhibition of the dsDNA pathway by RU.521 requires the presence of cGAS.

靶点：

DNA; cGAS;DNA; cGAS

体内研究：

Treatment of primary BMDMs with RU.521 or its analogs reduced IFNB1 expression in mice, indicating their effectiveness in suppressing intrinsic DNA-dependent, constitutively-activated type I interferon expression in cells deficient of a cytoplasmic DNA exonuclease.

细胞实验：

Small-molecule compounds were serially diluted to concentrations spanning the range tested in the response curves were added to 6.7 × 10^5 RAW-Blue macrophages plated 16h prior in 96-well dishes, then harvested 72h after compound addition. ATP was measured using CellTiter Glo Viability Assay using 50 μM Tamoxifen as a positive control for cytotoxicity. Viability values were generated using vehicle (DMSO) or the first dose as 100% and Tamoxifen as 0%. Outliers were removed.

动物实验：

the chronically elevated levels of cytokines observed in Trex1 null mice are a consequence of constitutively activated cGAS, due to the inability to eliminate aberrantly localized self-DNA. We harvested BMDMs from 6–8-week old Trex1 -/- mice, treated them with each compound, and measured expression levels of IFNB1 by quantitative reverse transcription PCR (qRT-PCR). Treatment of primary BMDMs with RU.521 or its analogs reduced IFNB1 expression, indicating their effectiveness in suppressing intrinsic DNA-dependent, constitutively-activated type I interferon expression in cells deficient of a cytoplasmic DNA exonuclease.

参考文献：

1. Vincent J, et al. Small molecule inhibition of cGAS reduces interferon expression in primary macrophages from autoimmune mice. Nat Commun. 2017 Sep 29;8(1):750.

溶解性：

Soluble in DMSO

保存条件：

-20℃

配置溶液浓度参考：

	1mg	5mg	10mg
1 mM	2.408 ml	12.042 ml	24.083 ml
5 mM	0.482 ml	2.408 ml	4.817 ml
10 mM	0.241 ml	1.204 ml	2.408 ml
50 mM	0.048 ml	0.241 ml	0.482 ml