• 产品描述： TED-347 是一种有效的、不可逆的 the TEAD⋅Yap protein-protein interaction 的共价变构抑制剂。TED-347 可抑制 TEAD4⋅Yap1 蛋白质相互作用，其EC50值为5.9 μM

• 靶点： TEAD4⋅Yap1(Cell-free assay):5.9 μM(EC50);YAP

• 体外研究：
  TED-347 is found to inhibit the protein-protein interaction in a time-dependent manner. TED-347 is shown to functionally disrupt the TEAD·Yap1 interaction in cells and to reduce the viability of patient-derived glioblastoma cell lines

• 细胞实验： Cell lines: HEK-293 cells, GBM43 cells Concentrations: 0.5 µM, 1.0 µM, 5.0 µM, 10 µM, 25 µM Incubation Time: 48 h Method: HEK-293 or GBM43 cells are plated at 2.43104 cells/well in a 96-well microplate and are transfected after 24 hr with the a pGL3.1 reporter containing the CTGF promoter and a plasmid encoding TK-Renilla luciferase in combination with control vectors or vectors that express Yap1 and TEAD4. After 48 hr, cells are treated with 0.5, 1.0, 5.0 or 10 µM of TED-347 for another 48 hr.

• 参考文献：
  1. Khuchtumur Bum-Erdene, et al. Small-Molecule Covalent Modification of Conserved Cysteine Leads to Allosteric Inhibition of the TEAD⋅Yap Protein-Protein Interaction. Cell Chem Biol. 2019 Mar 21;26(3):378-389.e13.

• 溶解性： Soluble  in  DMSO、Ethanol

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	3.188 ml	15.939 ml	31.878 ml
  5 mM	0.638 ml	3.188 ml	6.376 ml
  10 mM	0.319 ml	1.594 ml	3.188 ml
  50 mM	0.064 ml	0.319 ml	0.638 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *